Revzin's team collaborated with UCLA electrical engineer Prof. Aydogan Ozcan to integrate an antibody microarray with a lensfree holographic imaging device. The test returns results six to twelve times faster than traditional approaches and tests six parameters simultaneously. The lab on a chip device will have important applications in multiparametric blood analysis, especially in resource-poor areas for point of care analyses; nevertheless it will be also an attractive option in wealthier areas as a good and fast practice in clinical use. More than HIV testing and monitoring this will be a fast responsive device to guarantee safe blood transfusions.
The device technology breaktrough resides in the cheap and fast solution compared to the presently used flow cytometry supported by expensive devices run by skilled and trained specialists. The lab on a chip is capable of providing multiparametric screening (actually testing six parameters simultaneously) in few seconds with a minimal amount of blood. The test consists of polymer film imprinted with an array of miniature spots. Each spot contains antibodies specific to the two kinds of T-cells (CD4 and CD8) and three types of cytokines printed in the same array. When the blood flowed across the antibody spots, T cells stop and get stuck on the spots. Each T-cell type is captured next to antibody spots specific for the cytokines they produce. When antibodies activate the cells, spots adjacent to the cells capture the cytokines they secreted. This connects a specific T-cell subset to its secreted cytokines. The visible color intensity of antibody spots reveals differences in cytokine production by T-cells. The lensfree on-chip imaging developed by Prof. Aydogan Ozcan at UCLA allows to image in few seconds and to rapidly count T-cell arrays without the use of any lenses or mechanical scanning. The device will evolve by adding microarrays to test and detect proteins from HIV and Hepatitis C viruses.
The Revzin team published the results of their experiments in the May 2010 issue of Analytical Chemistry.
More information in: Gulnaz Stybayeva, Onur Mudanyali, Sungkyu Seo, Jaime Silangcruz, Monica Macal, Erlan Ramanculov, Satya Dandekar, Anthony Erlinger, Aydogan Ozcan and Alexander Revzin Anal. Chem., 2010, 82 (9), pp 3736–3744